Standard Article

Asymmetric Flow Field Flow Fractionation

Liquid Chromatography

  1. Stepan Podzimek

Published Online: 17 DEC 2012

DOI: 10.1002/9780470027318.a9289

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Podzimek, S. 2012. Asymmetric Flow Field Flow Fractionation. Encyclopedia of Analytical Chemistry. .

Author Information

  1. University of Pardubice, Pardubice, Czech Republic

Publication History

  1. Published Online: 17 DEC 2012

Abstract

The article provides overview of the theory and principles of asymmetric flow field flow fractionation (A4F). Effect of various operational parameters on the resolution of the A4F technique is discussed. For the sake of better understanding, the A4F method is contrasted with widely known size exclusion chromatography (SEC). In contrast to SEC, in which the size restricted permeation of macromolecules into the pores of different size is the major separation mechanism. A4F separates the molecules without contribution of a stationary phase. That is the most distinguished feature of the method, which offers several advantages over SEC. The focus is on the combination of A4F with a multiangle light scattering (MALS) detector, which allows direct determination of molar mass and size distribution and characterization of branching. Theoretical and descriptive part is accomplished by several examples of experimental results obtained by the measurements of typical polymers, nanoparticles, and proteins.