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Multidimensional Separations by Ion Mobility-Mass Spectrometry

Mass Spectrometry

  1. Kelly M. Hines,
  2. Jeffrey R. Enders,
  3. John A. McLean

Published Online: 17 DEC 2012

DOI: 10.1002/9780470027318.a9313

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Hines, K. M., Enders, J. R. and McLean, J. A. 2012. Multidimensional Separations by Ion Mobility-Mass Spectrometry. Encyclopedia of Analytical Chemistry. .

Author Information

  1. Vanderbilt University, Nashville, TN, USA

Publication History

  1. Published Online: 17 DEC 2012


Ion mobility-mass spectrometry (IM-MS) couples gas-phase structural separations by IM with mass-to-charge separations by MS. The utility of this integration of multidimensional separation dimensions is manifold, including (i) high throughput multidimensional separations, (ii) separation of chemical noise, and (iii) rapid information in structural biology. This article introduces the unique advantages of IM-MS in biological fields ranging from systems biology to structural biology, the context of which is introduced by a historical perspective of key advances accomplished over the last century. Contemporary and emerging technology for performing IM-MS is presented, and applications using conformational data sets in proteomics, glycomics, and lipidomics are described.