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High-Resolution 4D Imaging in Live Cells

Peptides and Proteins

  1. Maya Shamir,
  2. Daniel Kaganovich

Published Online: 15 MAR 2013

DOI: 10.1002/9780470027318.a9326

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Shamir, M. and Kaganovich, D. 2013. High-Resolution 4D Imaging in Live Cells. Encyclopedia of Analytical Chemistry. .

Author Information

  1. Hebrew University of Jerusalem, Department of Cell and Developmental Biology, Alexander Silberman Institute of Life Sciences, Jerusalem, Israel

Publication History

  1. Published Online: 15 MAR 2013


The technological sophistication of microscopy approaches has grown by leaps and bounds over the past decade. This has yielded microscopes capable of greater sensitivity, faster acquisition, and higher resolution, as well as fluorophores and imaging tools that demonstrate more and more diversity of properties and applications. Yet for all the putative improvements to the cell biologists' tool kit, the application of potentially invaluable tools to unsolved scientific problems is still in its infancy. The purpose of our review is to discuss important innovations in live-cell imaging, focusing on practical considerations for probing biological systems at the molecular level while remaining rooted in a live-cell context.