Chapter 4. Tubule Formation Assays

  1. Carolyn A. Staton2,3,
  2. Claire Lewis4 and
  3. Roy Bicknell5
  1. Ewen J. Smith1 and
  2. Carolyn A. Staton2,3

Published Online: 27 SEP 2007

DOI: 10.1002/9780470029350.ch4

Angiogenesis Assays: A Critical Appraisal of Current Techniques

Angiogenesis Assays: A Critical Appraisal of Current Techniques

How to Cite

Smith, E. J. and Staton, C. A. (2006) Tubule Formation Assays, in Angiogenesis Assays: A Critical Appraisal of Current Techniques (eds C. A. Staton, C. Lewis and R. Bicknell), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470029350.ch4

Editor Information

  1. 2

    Academic Unit of Surgical Oncology, University of Sheffield Medical School, Sheffield, UK

  2. 3

    Academic Unit of Surgical Oncology, K-Floor, Royal Hallamshire Hospital, Glossop Road, Sheffield S10 2JF, UK

  3. 4

    Academic Unit of Pathology, University of Sheffield Medical School, Sheffield, UK

  4. 5

    Division of Immunity and Infection, University of Birmingham, Birmingham, UK

Author Information

  1. 1

    Tumour Targeting Group, Academic Unit of Pathology, University of Sheffield, Sheffield, S10 2RX, UK

  2. 2

    Academic Unit of Surgical Oncology, University of Sheffield Medical School, Sheffield, UK

  3. 3

    Academic Unit of Surgical Oncology, K-Floor, Royal Hallamshire Hospital, Glossop Road, Sheffield S10 2JF, UK

Publication History

  1. Published Online: 27 SEP 2007
  2. Published Print: 15 DEC 2006

ISBN Information

Print ISBN: 9780470016008

Online ISBN: 9780470029350

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Keywords:

  • two-dimensional (2D) tubule assays;
  • tubule formation assays;
  • bovine aortic endothelial cells (BAEC);
  • primary human umbilical vein endothelial cells (HUVECs);
  • endothelial cell morphology and tubule formation;
  • tubule assay matrices;
  • microcarriers in fibrin gel;
  • matrix for 3D assays

Summary

This chapter contains sections titled:

  • Introduction

  • Endothelial cell sources

  • Endothelial cell morphology and tubule formation

  • Tubule assay matrices

  • 2D assay protocols

  • Analysis of tubule formation in 2D assays

  • Recent developments in 2D assays

  • 3D assays

  • Analysis of 3D assays

  • Co-culture assays

  • Conclusions

  • References