Chapter 5. Modelling the Effects of the Haemodynamic Environment on Endothelial Cell Responses Relevant to Angiogenesis

  1. Carolyn A. Staton2,3,
  2. Claire Lewis4 and
  3. Roy Bicknell5
  1. Gerard B. Nash and
  2. Stuart Egginton

Published Online: 27 SEP 2007

DOI: 10.1002/9780470029350.ch5

Angiogenesis Assays: A Critical Appraisal of Current Techniques

Angiogenesis Assays: A Critical Appraisal of Current Techniques

How to Cite

Nash, G. B. and Egginton, S. (2006) Modelling the Effects of the Haemodynamic Environment on Endothelial Cell Responses Relevant to Angiogenesis, in Angiogenesis Assays: A Critical Appraisal of Current Techniques (eds C. A. Staton, C. Lewis and R. Bicknell), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470029350.ch5

Editor Information

  1. 2

    Academic Unit of Surgical Oncology, University of Sheffield Medical School, Sheffield, UK

  2. 3

    Academic Unit of Surgical Oncology, K-Floor, Royal Hallamshire Hospital, Glossop Road, Sheffield S10 2JF, UK

  3. 4

    Academic Unit of Pathology, University of Sheffield Medical School, Sheffield, UK

  4. 5

    Division of Immunity and Infection, University of Birmingham, Birmingham, UK

Author Information

  1. Centre for Cardiovascular Sciences and Department of Physiology, The Medical School, The University of Birmingham, Birmingham B15 2TT, UK

Publication History

  1. Published Online: 27 SEP 2007
  2. Published Print: 15 DEC 2006

ISBN Information

Print ISBN: 9780470016008

Online ISBN: 9780470029350

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Keywords:

  • haemodynamic conditioning;
  • endothelial cells (EC) key angiogenic responses;
  • vascular endothelial growth factor-A (VEGF-A) expression;
  • endothelial cells and shear stress;
  • flow-based endothelial studies;
  • in vivo angiogenesis control;
  • influencing endothelial shear sensing;
  • ‘angiogenic’ responses of endothelial cells

Summary

This chapter contains sections titled:

  • Introduction

  • Definitions

  • Experimental patterns of shear exposure and relevance to angiogenesis assays

  • Methods for studying responses of endothelial cells exposed to shear stress

  • Readouts in flow cultures relevant to angiogenesis

  • Critical considerations and conclusions

  • References