Chapter 8. Assays for Membrane and Intracellular Signalling Events

  1. Carolyn A. Staton2,3,
  2. Claire Lewis4 and
  3. Roy Bicknell5
  1. Vittorio Tomasi,
  2. Cristiana Griffoni,
  3. Spartaco Santi,
  4. Patrizia Lenaz,
  5. Rosa Anna Iorio,
  6. Antonio Strillacci and
  7. Enzo Spisni

Published Online: 27 SEP 2007

DOI: 10.1002/9780470029350.ch8

Angiogenesis Assays: A Critical Appraisal of Current Techniques

Angiogenesis Assays: A Critical Appraisal of Current Techniques

How to Cite

Tomasi, V., Griffoni, C., Santi, S., Lenaz, P., Iorio, R. A., Strillacci, A. and Spisni, E. (2006) Assays for Membrane and Intracellular Signalling Events, in Angiogenesis Assays: A Critical Appraisal of Current Techniques (eds C. A. Staton, C. Lewis and R. Bicknell), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470029350.ch8

Editor Information

  1. 2

    Academic Unit of Surgical Oncology, University of Sheffield Medical School, Sheffield, UK

  2. 3

    Academic Unit of Surgical Oncology, K-Floor, Royal Hallamshire Hospital, Glossop Road, Sheffield S10 2JF, UK

  3. 4

    Academic Unit of Pathology, University of Sheffield Medical School, Sheffield, UK

  4. 5

    Division of Immunity and Infection, University of Birmingham, Birmingham, UK

Author Information

  1. Department of Experimental Biology, University of Bologna, Via Selmi 3, 40126 Bologna, Italy

Publication History

  1. Published Online: 27 SEP 2007
  2. Published Print: 15 DEC 2006

ISBN Information

Print ISBN: 9780470016008

Online ISBN: 9780470029350

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Keywords:

  • signalling protein complexes in membrane microdomains;
  • membrane and intracellular signalling events assays;
  • endothelial nitric oxide synthase (eNOS);
  • vascular endothelium growth factor (VEGF);
  • fluorescence resonance energy transfer (FRET);
  • chorioallantoic membrane (CAM) in vivo;
  • antisense and siRNA

Summary

This chapter contains sections titled:

  • Introduction

  • The endothelial caveolae as the organizers of efficient spatial signal transduction mechanisms

  • The prostaglandins forming system: regulation of signal transduction and angiogenesis

  • Use of antisense oligo and siRNA to evaluate the function of single signalling molecules

  • Knocking down and knocking out Caveolin-1 gene

  • Analysing protein-protein interactions in signalling molecules involved in angiogenesis

  • Final remarks

  • References