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Plant Metabolomics

  1. Gregory A. Barding Jr,
  2. Daniel J. Orr,
  3. Cynthia K. Larive

Published Online: 15 DEC 2011

DOI: 10.1002/9780470034590.emrstm1267

eMagRes

eMagRes

How to Cite

Barding, G. A., Orr, D. J. and Larive, C. K. 2011. Plant Metabolomics. eMagRes. .

Author Information

  1. University of California, Riverside, CA, USA

Publication History

  1. Published Online: 15 DEC 2011

Abstract

Plant metabolomics is a growing area of research providing important information about metabolic regulation by plants as a result of stress, genetic variation, or environment. Owing to the highly diverse and complex nature of plant metabolism, careful consideration of the analytical approach to plant metabolomics is important. This article reviews nuclear magnetic resonance (NMR) techniques for collection of metabolomic data from plant tissue including quantitative 2-D NMR and hyphenated NMR methods for sample enrichment and class-specific analysis. An overview of experimental design considerations, data normalization methods, and sample preparation strategies is provided. Finally, progress in the application of metabolic flux experiments and MRI toward understanding the plant metabolome is discussed.

Keywords:

  • plant metabolomics;
  • NMR spectroscopy;
  • systems biology;
  • sample preparation;
  • metabolic flux;
  • plant MRI;
  • solid-phase extraction;
  • liquid chromatography;
  • metabolite profiling;
  • normalization