Unit

UNIT 2.4 Fluorescence Spectroscopy

  1. Claudia Y. Lee

Published Online: 1 DEC 2009

DOI: 10.1002/9780470089941.et0204s02

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Lee, C. Y. 2009. Fluorescence Spectroscopy. Current Protocols Essential Laboratory Techniques. 2:2.4:2.4.1–2.4.29.

Author Information

  1. UVP, LLC, Upland, California

Publication History

  1. Published Online: 1 DEC 2009
  2. Published Print: DEC 2009

Abstract

Fluorescence is an extremely powerful tool in modern biology, physics, and chemistry laboratories. This unit focuses on methods that can be applied to biological studies. Starting with the preparation of fluorescently conjugated antibodies, the first part explains the physical principles of fluorescence and the features in an absorption spectrum, and how these are used to measure the degree of labeling on conjugated antibodies. The protocols describe how to obtain and interpret a fluorescence emission spectrum and utilize this information as a fluorescence indicator for protein detection. Curr. Protoc. Essential Lab. Tech. 2:2.4.1-2.4.29. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • fluorescence;
  • immunolabeling;
  • absorption spectrum;
  • emission spectrum;
  • fluorometer;
  • spectrometer;
  • steady state anisotropy