UNIT 3.1 Reagent Preparation: Theoretical and Practical Discussions

  1. Deb N. Chakravarti,
  2. Bulbul Chakravarti,
  3. Buddhadeb Mallik

Published Online: 1 OCT 2008

DOI: 10.1002/9780470089941.et0301s00

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Chakravarti, D. N., Chakravarti, B. and Mallik, B. 2008. Reagent Preparation: Theoretical and Practical Discussions. Current Protocols Essential Laboratory Techniques. 00:3.1:3.1.1–3.1.13.

Author Information

  1. Proteomics Center, Keck Graduate Institute of Applied Life Sciences, Claremont, California

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: JAN 2008

This is not the most recent version of the article. View current version (28 OCT 2014)


One of the most important requirements for the successful completion of any biochemical protocol is to prepare reagents accurately. This unit describes general guidelines for the preparation of reagents, use of clean glass- and plasticware, use of high-purity reagents, safe handling of chemicals and biochemicals, use of high-purity distilled or deionized water, accurate weighing, pipetting, and pH measurement, and avoidance of chemical or microbial contamination of reagents and use of sterile reagents. Different units of concentration used for preparation of reagents or buffer solutions are described. This unit also includes a section for the preparation of buffer solutions with a practical example.


  • biochemicals;
  • buffer;
  • chemicals;
  • deionized water;
  • distilled water;
  • glassware;
  • pH;
  • plastic ware;
  • reagents;
  • units of concentration