UNIT 3.2 Measurement of pH

  1. Emily A. Wiley1,
  2. Deb N. Chakravarti2,
  3. Kristin M. Slade1

Published Online: 1 JUN 2010

DOI: 10.1002/9780470089941.et0302s03

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Wiley, E. A., Chakravarti, D. N. and Slade, K. M. 2010. Measurement of pH. Current Protocols Essential Laboratory Techniques. 3:3.2:3.2.1–3.2.16.

Author Information

  1. 1

    Joint Science Department, Claremont McKenna, Pitzer, and Scripps Colleges, Claremont, California

  2. 2

    Proteomics Center, Keck Graduate Institute of Applied Life Sciences, Claremont, California

Publication History

  1. Published Online: 1 JUN 2010
  2. Published Print: JUN 2010


The measurement of pH is one of the most basic and necessary skills in a life science laboratory. The function and physical characteristics of biological molecules are highly sensitive to the pH of the environment. Common biological buffers must be prepared with the appropriate pH, usually close to neutral for most biological applications. This unit includes a discussion of the different pH instrumentation, notably pH electrodes. It also relates basic pH measurement theory to critical parameters for the technique, and covers the correct use, handling, and storage of pH instruments. Curr. Protoc. Essential Lab. Tech. 3:3.2.1-3.2.16. © 2010 by John Wiley & Sons, Inc.


  • electrode;
  • meter;
  • calibration;
  • Nernst;
  • electrical potential