UNIT 4.1 Aseptic Technique

  1. Tomasz Bykowski,
  2. Brian Stevenson

Published Online: 1 OCT 2008

DOI: 10.1002/9780470089941.et0401s00

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Bykowski, T. and Stevenson, B. 2008. Aseptic Technique. Current Protocols Essential Laboratory Techniques. 00:4.1:4.1.1–4.1.11.

Author Information

  1. University of Kentucky College of Medicine, Lexington, Kentucky

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: JAN 2008


This unit describes common laboratory procedures that can reduce the risk of culture contamination (sepsis), collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on Petri dishes.


  • sterile technique;
  • sterile work;
  • Bunsen burner;
  • laminar flow;
  • laminar hood