Unit

UNIT 4.1 Aseptic Technique

  1. Tomasz Bykowski,
  2. Brian Stevenson

Published Online: 1 OCT 2008

DOI: 10.1002/9780470089941.et0401s00

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Bykowski, T. and Stevenson, B. 2008. Aseptic Technique. Current Protocols Essential Laboratory Techniques. 00:4.1:4.1.1–4.1.11.

Author Information

  1. University of Kentucky College of Medicine, Lexington, Kentucky

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: JAN 2008

Abstract

This unit describes common laboratory procedures that can reduce the risk of culture contamination (sepsis), collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on Petri dishes.

Keywords:

  • sterile technique;
  • sterile work;
  • Bunsen burner;
  • laminar flow;
  • laminar hood