Unit

UNIT 5.1 Centrifugation

  1. Sean R. Gallagher

Published Online: 1 OCT 2008

DOI: 10.1002/9780470089941.et0501s00

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Gallagher, S. R. 2008. Centrifugation. Current Protocols Essential Laboratory Techniques. 00:5.1:5.1.1–5.1.16.

Author Information

  1. UVP, LLC, Upland, California

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: JAN 2008

This is not the most recent version of the article. View current version (28 OCT 2014)

Abstract

Centrifugation is a ubiquitous technique in the life science laboratory, and uses specialized equipment (centrifuges) to spin or centrifuge samples in rotors, generating centrifugal force for a range of applications that include pelleting of precipitates, isolating organelles, and protein and DNA purification. The unit describes basic applications, definitions, and references, and provides up to date tables for rotor specifications from major suppliers.

Keywords:

  • centrifuge;
  • high speed centrifuge;
  • ultracentrifuge;
  • analytical ultracentrifuge;
  • DNA;
  • protein;
  • cell fractionation;
  • rotor;
  • g force;
  • organelle;
  • k factor