Unit

UNIT 6.1 Overview of Chromatography

  1. Buddhadeb Mallik,
  2. Bulbul Chakravarti,
  3. Deb N. Chakravarti

Published Online: 1 OCT 2008

DOI: 10.1002/9780470089941.et0601s00

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Mallik, B., Chakravarti, B. and Chakravarti, D. N. 2008. Overview of Chromatography. Current Protocols Essential Laboratory Techniques. 00:6.1:6.1.1–6.1.22.

Author Information

  1. Proteomics Center, Keck Graduate Institute of Applied Life Sciences, Claremont, California

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: JAN 2008

Abstract

Chromatography in its many different forms is an indispensable separation technique for purification or analysis of one or multiple components from a mixture that may contain a variety of simple as well as complex molecules. This unit is focused on a specific type of chromatography known as liquid chromatography (LC), with particular reference to separation of proteins. Theory of chromatography is discussed in the context of partition of the components of a mixture between a solid stationary phase and a liquid mobile phase. Although very useful for chromatographic separation of small molecules, there is, however, considerable deviation observed in the case of separation of proteins. Principles of three commonly used chromatographic techniques for separation of proteins, such as size exclusion, ion exchange, and affinity chromatography are discussed. The unit is expected to be useful for biologists and biochemists, as well as engineers.

Keywords:

  • chromatography;
  • liquid chromatography;
  • theory of chromatography;
  • plate model;
  • size exclusion chromatography;
  • gel filtration;
  • ion exchange chromatography;
  • affinity chromatography