UNIT 6.1 Overview of Chromatography
Published Online: 1 OCT 2008
Copyright © 2008 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols Essential Laboratory Techniques
How to Cite
Mallik, B., Chakravarti, B. and Chakravarti, D. N. 2008. Overview of Chromatography. Current Protocols Essential Laboratory Techniques. 00:6.1:6.1.1–6.1.22.
- Published Online: 1 OCT 2008
- Published Print: JAN 2008
Chromatography in its many different forms is an indispensable separation technique for purification or analysis of one or multiple components from a mixture that may contain a variety of simple as well as complex molecules. This unit is focused on a specific type of chromatography known as liquid chromatography (LC), with particular reference to separation of proteins. Theory of chromatography is discussed in the context of partition of the components of a mixture between a solid stationary phase and a liquid mobile phase. Although very useful for chromatographic separation of small molecules, there is, however, considerable deviation observed in the case of separation of proteins. Principles of three commonly used chromatographic techniques for separation of proteins, such as size exclusion, ion exchange, and affinity chromatography are discussed. The unit is expected to be useful for biologists and biochemists, as well as engineers.
- liquid chromatography;
- theory of chromatography;
- plate model;
- size exclusion chromatography;
- gel filtration;
- ion exchange chromatography;
- affinity chromatography