UNIT 6.2 Column Chromatography

  1. Bulbul Chakravarti,
  2. Buddhadeb Mallik,
  3. Deb N. Chakravarti

Published Online: 1 OCT 2008

DOI: 10.1002/9780470089941.et0602s00

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Chakravarti, B., Mallik, B. and Chakravarti, D. N. 2008. Column Chromatography. Current Protocols Essential Laboratory Techniques. 00:6.2:6.2.1–6.2.14.

Author Information

  1. Proteomics Center, Keck Graduate Institute of Applied Life Sciences, Claremont, California

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: JAN 2008

This is not the most recent version of the article. View current version (1 NOV 2016)


Chromatography is a widely used technique of separation which depends on the differential interaction of molecules between a stationary phase and a mobile phase. In most modern life science laboratories, purification of proteins plays an important role. Almost all types of laboratory-based liquid chromatography techniques used for purification of proteins are carried out using the stationary phase contained in tubular columns. Although individual methods for sample loading or elution of these columns are usually different depending on the specific technique used, this general experimental procedure that uses tubular columns is known as column chromatography. This unit is focused on setting up laboratory-based column chromatography methods for separation of proteins using three commonly used chromatographic techniques for separation of proteins, such as size exclusion, ion exchange, and affinity chromatography.


  • chromatography;
  • liquid chromatography;
  • column chromatography;
  • size exclusion chromatography;
  • gel filtration chromatography;
  • ion exchange chromatography;
  • affinity chromatography