UNIT 6.3 Thin Layer Chromatography

  1. Li Cai

Published Online: 11 FEB 2014

DOI: 10.1002/9780470089941.et0603s08

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Cai, L. 2014. Thin Layer Chromatography. Current Protocols Essential Laboratory Techniques. 6:6.3:6.3.1–6.3.18.

Author Information

  1. Division of Mathematics and Science, University of South Carolina Salkehatchie, Walterboro, South Carolina

Publication History

  1. Published Online: 11 FEB 2014


Thin layer chromatography (TLC) is a quick, sensitive, and inexpensive technique used to determine the number of components in a mixture, verify the identity and purity of a compound, monitor the progress of a reaction, determine the solvent composition for preparative separations, and analyze the fractions obtained from column chromatography. This unit is mainly aimed at novice experimenters, describing in detail the strategies and principal steps for performing a TLC experiment, with illustrations of the relevant instruments, as well as approaches for obtaining and understanding results. Valuable practical tips and troubleshooting solutions are also provided throughout the unit. Curr. Protoc. Essential Lab. Tech. 8:6.3.1-6.3.18. © 2014 by John Wiley & Sons, Inc.


  • chromatography;
  • TLC;
  • separation;
  • silica gel;
  • organic solvents;
  • staining