UNIT 7.4 Staining Proteins in Gels
Published Online: 1 OCT 2008
Copyright © 2008 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols Essential Laboratory Techniques
How to Cite
Gallagher, S. R. and Sasse, J. 2008. Staining Proteins in Gels. Current Protocols Essential Laboratory Techniques. 00:7.4:7.4.1–7.4.11.
- Published Online: 1 OCT 2008
- Published Print: JAN 2008
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This unit describes protocols for detecting proteins in SDS-polyacrylamide gels. It describes methods for Coomassie blue and silver staining, as well as the fluorescent stains SYPRO orange and red. Staining with Coomassie blue is easier and more rapid; however, silver staining methods are considerably more sensitive and can thus be used to detect smaller amounts of protein. Alternative rapid staining procedures are provided for each method. Fluorescent staining is a popular alternative to the traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining.