Unit

UNIT 7.4 Staining Proteins in Gels

  1. Sean R. Gallagher1,
  2. Joachim Sasse2

Published Online: 1 OCT 2008

DOI: 10.1002/9780470089941.et0704s00

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Gallagher, S. R. and Sasse, J. 2008. Staining Proteins in Gels. Current Protocols Essential Laboratory Techniques. 00:7.4:7.4.1–7.4.11.

Author Information

  1. 1

    UVP, LLC, Upland, California

  2. 2

    Shriners Hospital for Crippled Children, Tampa, Florida

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: JAN 2008

This is not the most recent version of the article. View current version (1 AUG 2012)

Abstract

This unit describes protocols for detecting proteins in SDS-polyacrylamide gels. It describes methods for Coomassie blue and silver staining, as well as the fluorescent stains SYPRO orange and red. Staining with Coomassie blue is easier and more rapid; however, silver staining methods are considerably more sensitive and can thus be used to detect smaller amounts of protein. Alternative rapid staining procedures are provided for each method. Fluorescent staining is a popular alternative to the traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining.

Keywords:

  • silver;
  • Coomassie;
  • stain;
  • protein;
  • fluorescence;
  • electrophoresis;
  • SYPRO