UNIT 7.4 Staining Proteins in Gels
Published Online: 1 AUG 2012
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols Essential Laboratory Techniques
How to Cite
Gallagher, S. R. and Sasse, J. 2012. Staining Proteins in Gels. Current Protocols Essential Laboratory Techniques. 6:7.4:7.4.1–7.4.14.
- Published Online: 1 AUG 2012
This unit describes protocols for detecting proteins in SDS-polyacrylamide gels. It describes methods for Coomassie blue and silver staining, as well as the fluorescent stains SYPRO Orange and Red. Staining with Coomassie blue is easier and more rapid; however, silver staining methods are considerably more sensitive and can thus be used to detect smaller amounts of protein. Alternative rapid staining procedures are provided for each method. Fluorescent staining is a popular alternative to the traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining. Curr. Protoc. Essential Lab. Tech. 6:7.4.1-7.4.14. © 2012 by John Wiley & Sons, Inc.