Unit

UNIT 7.4 Staining Proteins in Gels

  1. Sean R. Gallagher1,
  2. Joachim Sasse2

Published Online: 1 AUG 2012

DOI: 10.1002/9780470089941.et0704s06

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Gallagher, S. R. and Sasse, J. 2012. Staining Proteins in Gels. Current Protocols Essential Laboratory Techniques. 6:7.4:7.4.1–7.4.14.

Author Information

  1. 1

    UVP, LLC, Upland, California

  2. 2

    Biomedtech Laboratories, Inc., Tampa, Florida

Publication History

  1. Published Online: 1 AUG 2012

Abstract

This unit describes protocols for detecting proteins in SDS-polyacrylamide gels. It describes methods for Coomassie blue and silver staining, as well as the fluorescent stains SYPRO Orange and Red. Staining with Coomassie blue is easier and more rapid; however, silver staining methods are considerably more sensitive and can thus be used to detect smaller amounts of protein. Alternative rapid staining procedures are provided for each method. Fluorescent staining is a popular alternative to the traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining. Curr. Protoc. Essential Lab. Tech. 6:7.4.1-7.4.14. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • silver;
  • Coomassie;
  • stain;
  • protein;
  • fluorescence;
  • electrophoresis;
  • SYPRO