UNIT 7.5 Overview of Digital Electrophoresis Analysis
Published Online: 1 OCT 2008
Copyright © 2008 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols Essential Laboratory Techniques
How to Cite
Moomaw, B., Medberry, S. and Gallagher, S. R. 2008. Overview of Digital Electrophoresis Analysis. Current Protocols Essential Laboratory Techniques. 00:7.5:7.5.1–7.5.29.
- Published Online: 1 OCT 2008
- Published Print: JAN 2008
This is not the most recent version of the article. View current version (28 OCT 2014)
Digital imaging is the method of choice for documentation and analysis of electrophoretic separations of protein and DNA. Digital images of gel electropherograms can be obtained rapidly using CCD-based cameras, and the images can be easily archived and analyzed using image analysis software. This overview defines important key terms and calculations for imaging, explains the capture process, reviews the range of CCD technologies used for image capture, and provides an introduction to the software and methods used for one- and two-dimensional digital image analysis.
- digital analysis;