Unit

UNIT 7.5 Overview of Digital Electrophoresis Analysis

  1. Butch Moomaw1,
  2. Scott Medberry2,
  3. Sean R. Gallagher3

Published Online: 1 OCT 2008

DOI: 10.1002/9780470089941.et0705s00

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Moomaw, B., Medberry, S. and Gallagher, S. R. 2008. Overview of Digital Electrophoresis Analysis. Current Protocols Essential Laboratory Techniques. 00:7.5:7.5.1–7.5.29.

Author Information

  1. 1

    Hamamatsu Photonic Systems, Spring Branch, Texas

  2. 2

    Agilent Technologies, Inc., Palo Alto, California

  3. 3

    UVP, LLC, Upland, California

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: JAN 2008

This is not the most recent version of the article. View current version (28 OCT 2014)

Abstract

Digital imaging is the method of choice for documentation and analysis of electrophoretic separations of protein and DNA. Digital images of gel electropherograms can be obtained rapidly using CCD-based cameras, and the images can be easily archived and analyzed using image analysis software. This overview defines important key terms and calculations for imaging, explains the capture process, reviews the range of CCD technologies used for image capture, and provides an introduction to the software and methods used for one- and two-dimensional digital image analysis.

Keywords:

  • electrophoresis;
  • imaging;
  • software;
  • digital analysis;
  • quantitation