Unit

UNIT 8.1 Overview of Blotting

  1. Maria Cristina Negritto1,
  2. Glenn M. Manthey2

Published Online: 1 OCT 2008

DOI: 10.1002/9780470089941.et0801s00

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Negritto, M. C. and Manthey, G. M. 2008. Overview of Blotting. Current Protocols Essential Laboratory Techniques. 00:8.1:8.1.1–8.1.19.

Author Information

  1. 1

    Pomona College, Claremont, California

  2. 2

    City of Hope, Beckman Research Institute, Duarte, California

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: JAN 2008

This is not the most recent version of the article. View current version (1 NOV 2016)

Abstract

Blotting techniques are among the most common approaches used in a molecular biology laboratory. These techniques, Southern, northern, and immunoblotting, are applicable to a variety of macromolecules including DNA, RNA, and protein, respectively. Each of the techniques are dependent on the ability to resolve the individual macromolecules in a size-dependant manner, transfer the molecules to a solid support, and finally use a defined probe to detect the specific molecule of interest. The utilization of the blotting technology over the last 30 years has been instrumental to the elucidation of many fundamental biological processes. The continued use of blotting technology holds promise for even greater discovery over the next 30 years.

Keywords:

  • Southern;
  • Northern;
  • Western;
  • Probe;
  • Hybridization;
  • antibody;
  • membrane;
  • blotting;
  • protein blot;
  • DNA blot;
  • RNA blot