UNIT 9.1 Conventional Light Microscopy

  1. Eric S. Cole

Published Online: 1 OCT 2008

DOI: 10.1002/9780470089941.et0901s00

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Cole, E. S. 2008. Conventional Light Microscopy. Current Protocols Essential Laboratory Techniques. 00:9.1:9.1.1–9.1.28.

Author Information

  1. St. Olaf College, Northfield, Minnesota

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: JAN 2008

This is not the most recent version of the article. View current version (2 MAY 2016)


In this molecular day and age, microscopy seems to be a neglected field of instruction. Too often professors, who themselves are strangers to the use of the light microscope, may hurry through a laboratory exercise designed to familiarize students with its uses. This chapter is designed to serve as a useful reference for instructors, perhaps demystifying some features of the microscope, and making their applications more user-friendly and exciting.


  • microscopy;
  • lens;
  • magnification;
  • focus;
  • resolution;
  • refraction;
  • phase-contrast