Unit

UNIT 10.1 Working with Enzymes

  1. David Skrincosky,
  2. Cindy Santangelo

Published Online: 1 OCT 2008

DOI: 10.1002/9780470089941.et1001s00

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Skrincosky, D. and Santangelo, C. 2008. Working with Enzymes. Current Protocols Essential Laboratory Techniques. 00:10.1:10.1.1–10.1.33.

Author Information

  1. Worthington Biochemical, Lakewood, New Jersey

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: JAN 2008

Abstract

This unit briefly describes the history, theory, and biochemical principles of enzymes. The enzyme classification system, which is based upon the reactions enzymes catalyze, is explained. Several factors affecting enzyme activity including concentration, temperature, pH, and substrate choices are reviewed. While it is difficult to make generalizations since each enzyme is unique, we have compiled useful tips on enzyme storage and commonly encountered forms. This unit also applies the basic general principles of enzymes to a specific class of enzymes, called restriction endonucleases, in order to demonstrate how to correctly set up an enzymatic reaction.

Keywords:

  • enzyme;
  • units;
  • classification;
  • kinetics;
  • inhibition;
  • specificity;
  • restriction endonucleases