UNIT 10.1 Working with Enzymes
Published Online: 1 OCT 2008
Copyright © 2008 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols Essential Laboratory Techniques
How to Cite
Skrincosky, D. and Santangelo, C. 2008. Working with Enzymes. Current Protocols Essential Laboratory Techniques. 00:10.1:10.1.1–10.1.33.
- Published Online: 1 OCT 2008
- Published Print: JAN 2008
This unit briefly describes the history, theory, and biochemical principles of enzymes. The enzyme classification system, which is based upon the reactions enzymes catalyze, is explained. Several factors affecting enzyme activity including concentration, temperature, pH, and substrate choices are reviewed. While it is difficult to make generalizations since each enzyme is unique, we have compiled useful tips on enzyme storage and commonly encountered forms. This unit also applies the basic general principles of enzymes to a specific class of enzymes, called restriction endonucleases, in order to demonstrate how to correctly set up an enzymatic reaction.
- restriction endonucleases