Unit

UNIT 12.1 DNA Sequencing: An Outsourcing Guide

  1. Jeffrey W. Touchman

Published Online: 1 DEC 2009

DOI: 10.1002/9780470089941.et1201s02

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Touchman, J. W. 2009. DNA Sequencing: An Outsourcing Guide. Current Protocols Essential Laboratory Techniques. 2:12.1:12.1.1–12.1.19.

Author Information

  1. Arizona State University, Tempe, Arizona

Publication History

  1. Published Online: 1 DEC 2009
  2. Published Print: DEC 2009

Abstract

DNA sequencing, the process of determining the precise order of nucleotides in a DNA strand, is a fundamental and ubiquitous method in molecular biology. Examples of its many uses include characterization of unknown DNA, mutation detection, allele identification, and clone construct confirmation. Advances in sequencing technology, motivated by the successful International Human Genome Project, have resulted in unprecedented access to high-quality and cost-effective automated analysis. It is increasingly common for researchers to delegate routine DNA sequencing needs to dedicated laboratories or companies specializing in this technology. In this unit, we discuss key protocols for maximizing the success of DNA sequencing when working with such service providers and describe detailed troubleshooting advice for analyzing anomalous results. Curr. Protoc. Essential Lab. Tech. 2:12.1.1-12.1.19. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • DNA sequencing;
  • capillary electrophoresis;
  • PCR cleanup;
  • plasmid preparation;
  • chromatogram