Unit

UNIT 12.2 Mass Spectrometry: An Outsourcing Guide

  1. Leroi V. DeSouza1,
  2. K.W. Michael Siu1,
  3. Ronald E. Pearlman2

Published Online: 1 DEC 2009

DOI: 10.1002/9780470089941.et1202s02

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

DeSouza, L. V., Siu, K. M. and Pearlman, R. E. 2009. Mass Spectrometry: An Outsourcing Guide. Current Protocols Essential Laboratory Techniques. 2:12.2:12.2.1–12.2.18.

Author Information

  1. 1

    Centre for Research in Mass Spectrometry and Department of Chemistry, York University, Toronto, Ontario, Canada

  2. 2

    Centre for Research in Mass Spectrometry and Department of Biology, York University, Toronto, Ontario, Canada

Publication History

  1. Published Online: 1 DEC 2009
  2. Published Print: DEC 2009

Abstract

Proteomics is the study of the proteins expressed from the genome of a cell or organism. Analytical mass spectrometry has recently become a powerful tool to study a cell or organism's proteome and is the focus of this chapter. Mass spectrometry can be used for both qualitative and quantitative analysis of proteomes. This chapter will focus on qualitative analysis, addressing questions of what proteins are present in a proteome and what post-translational modifications may be associated with these proteins. The instrumentation required for mass spectrometric analysis is generally not available in a standard research laboratory or as part of an undergraduate laboratory, being associated in general with a core facility. We will not describe here details of specific operation of the instruments. Here we focus on the common types of analysis presently in routine use and on preparation of samples for routine biological mass spectrometric analysis that will allow most laboratories, including undergraduate and graduate teaching laboratories, to prepare and analyze samples in experiments designed to obtain proteomic information. Curr. Protoc. Essential Lab. Tech. 2:12.2.1-12.1.18. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • mass spectrometry;
  • polyacrylamide gel electrophoresis;
  • electrospray ionization;
  • matrix assisted laser desorption ionization;
  • collision induced dissociation;
  • time of flight;
  • tandem mass spectrometry