APPENDIX 3C Digital Image Processing and Analysis with ImageJ

  1. Sean R. Gallagher

Published Online: 1 JUN 2010

DOI: 10.1002/9780470089941.eta03cs03

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

How to Cite

Gallagher, S. R. 2010. Digital Image Processing and Analysis with ImageJ. Current Protocols Essential Laboratory Techniques. 3:3C:A.3C.1–A.3C.24.

Author Information

  1. UVP, LLC, Upland, California

Publication History

  1. Published Online: 1 JUN 2010
  2. Published Print: JUN 2010

This is not the most recent version of the article. View current version (28 OCT 2014)


ImageJ is a freely available, cross-platform (e.g., Windows, Mac, Linux) image processing and analysis program developed by the NIH. In addition to being readily available for no cost, ImageJ is supported by a wide range of constantly evolving user-created functionalities to address a remarkable range of applications, complementing commercial software that typically comes with imaging instruments such as digital gel-imaging systems or microscopy workstations. New processing/analysis macros and plug-ins are routinely added to the support site, and are frequently validated via refereed publications. With the continued improvements and growth of fluorescence-based applications, ImageJ continues to be a mainstay in the laboratory. ImageJ has extensive support materials available online, its base code is regularly updated, and a survey of Medline references indicates that it is one of the most widely used image-analysis packages available today. Curr. Protoc. Essential Lab. Tech. 3:A.3C.1-A.3C.24. © 2010 by John Wiley & Sons, Inc.


  • computer graphics;
  • image enhancement;
  • image interpretation;
  • imaging;
  • microscopy;
  • software;
  • electrophoresis;
  • gel analysis;
  • fluorescence-based imaging;
  • digital imaging;
  • protein blotting