Chapter 5. Transgenic Technology and Its Role in Understanding Normal and Abnormal Mammalian Development

  1. Patrizia Ferretti2,
  2. Andrew Copp2,
  3. Cheryll Tickle3 and
  4. Gudrun Moore4
  1. Valerie Vidal and
  2. Andreas Schedl

Published Online: 25 JAN 2010

DOI: 10.1002/9780470090121.ch5

Embryos, Genes and Birth Defects, Second Edition

Embryos, Genes and Birth Defects, Second Edition

How to Cite

Vidal, V. and Schedl, A. (2008) Transgenic Technology and Its Role in Understanding Normal and Abnormal Mammalian Development, in Embryos, Genes and Birth Defects, Second Edition (eds P. Ferretti, A. Copp, C. Tickle and G. Moore), John Wiley & Sons Ltd, Oxford, UK. doi: 10.1002/9780470090121.ch5

Editor Information

  1. 2

    Neural Development Unit/Developmental Biology Unit, UCL Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK

  2. 3

    Division of Cell and Developmental Biology, School of Life Sciences, MSI/WTB Complex, University of Dundee, Dow Street, Dundee, UK

  3. 4

    Clinical and Molecular Genetics, UCL Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK

Author Information

  1. INSERM U636, Centre de Biochimie, Parc Valrose, 06108 Nice, France

Publication History

  1. Published Online: 25 JAN 2010
  2. Published Print: 21 NOV 2008

ISBN Information

Print ISBN: 9780470090107

Online ISBN: 9780470090121

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Keywords:

  • transgenic technology - role in normal and abnormal mammalian development;
  • transgenic technologies;
  • ectopic expression studies using heterologous promoter;
  • site-specific recombinases - for conditional gene targeting and chromosome engineering;
  • Cre–loxP system - activating genes in tissue-specific manner;
  • establishment of totipotent embryonic stem (ES) cell lines;
  • gene targeting - tool in gene function analysis;
  • point mutations - reduced activity of protein, creating ‘hypomorphic alleles’;
  • thymidine kinase gene (TK);
  • chromosome engineering - versatility of Cre—lox system

Summary

This chapter contains sections titled:

  • Introduction

  • Transgenic mice

  • Genetic manipulation using gene targeting in ES cells

  • Outlook and future developments

  • References