Chapter 4. Microscopic Analytical Methods for Fungi

  1. Chin S. Yang Ph.D.3 and
  2. Patricia A. Heinsohn Ph.D. CIH4
  1. De-Wei Li Ph.D.1,
  2. Chin S. Yang Ph.D.3 and
  3. F. Harrington Ph.D.2

Published Online: 1 AUG 2006

DOI: 10.1002/9780470112434.ch4

Sampling and Analysis of Indoor Microorganisms

Sampling and Analysis of Indoor Microorganisms

How to Cite

Li, D.-W., Yang, C. S. and Harrington, F. (2007) Microscopic Analytical Methods for Fungi, in Sampling and Analysis of Indoor Microorganisms (eds C. S. Yang and P. A. Heinsohn), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470112434.ch4

Editor Information

  1. 3

    P&K Microbiology Services, Inc., 1936 Olney Ave, Cherry Hill, NJ 08003, USA

  2. 4

    Micro Bios, 2309 Palmetto Avenue, C-1, Pacifica, CA 94044, USA

Author Information

  1. 1

    The Connecticut Agricultural Experiment Station, Valley Laboratory, 153 Cook Hill Road, P.O. Box 248, Windsor, CT 06095, Canada

  2. 2

    15 Hinman Street, Meriden, CT 06450, UK

  3. 3

    P&K Microbiology Services, Inc., 1936 Olney Ave, Cherry Hill, NJ 08003, USA

Publication History

  1. Published Online: 1 AUG 2006
  2. Published Print: 16 FEB 2007

ISBN Information

Print ISBN: 9780471730934

Online ISBN: 9780470112434

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Keywords:

  • microscopic analytical methods for fungi;
  • aseptic technique and biosafety;
  • spore count analysis techniques

Summary

This chapter contains sections titled:

  • Introduction

  • Principles and Usage of Microscopes

  • Aseptic Technique and Biosafety

  • Sample Preparation for Spore Count

  • Materials Needed for Preparing Samples

  • Staining and Mounting Techniques

  • Procedures for Identification and Quantification of Spore Traps

  • Techniques for Spore Count Analysis

  • Background Particulates (Nonspore Miscellaneous Materials)

  • Limits of Detection

  • Data Presentation

  • Variation of Replications and Duplications

  • Sample Preparation for Direct Examination or from Cultures

  • Evaluation of Fungal Infestation

  • Training of Microscopy Analysts

  • Quality Assurance/Quality Control Procedures

  • WEB Resources