3. Probing Protein Interactions Using Hydrogen–Deuterium Exchange Mass Spectrometry

  1. Kevin M. Downard
  1. David D. Weis1,
  2. Suma Kaveti1,2,
  3. Yan Wu1 and
  4. John R. Engen1

Published Online: 7 DEC 2006

DOI: 10.1002/9780470146330.ch3

Mass Spectrometry of Protein Interactions

Mass Spectrometry of Protein Interactions

How to Cite

Weis, D. D., Kaveti, S., Wu, Y. and Engen, J. R. (2007) Probing Protein Interactions Using Hydrogen–Deuterium Exchange Mass Spectrometry, in Mass Spectrometry of Protein Interactions (ed K. M. Downard), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470146330.ch3

Editor Information

  1. School of Molecular and Microbial Biosciences, The University of Sydney, Australia

Author Information

  1. 1

    Department of Chemistry, University of New Mexico, Albuquerque, NM 87131-0001, USA

  2. 2

    Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA

Publication History

  1. Published Online: 7 DEC 2006
  2. Published Print: 26 JUL 2007

ISBN Information

Print ISBN: 9780471793731

Online ISBN: 9780470146330

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Keywords:

  • probing protein interactions with hydrogen–deuterium exchange mass spectrometry;
  • protein interactions - electrostatic or hydrophobic;
  • coupling hydrogen exchange (HX) with mass spectrometry (MS)

Summary

This chapter contains sections titled:

  • Introduction

  • Hydrogen Exchange Background

  • General HX-MS Method

  • Interactions of Proteins

  • Examples

  • Conclusions

  • Acknowledgements

  • References