6. Genesis and Application of Radical Probe Mass Spectrometry (RP-MS) to Study Protein Interactions

  1. Kevin M. Downard
  1. Simin D. Maleknia1 and
  2. Kevin M. Downard2

Published Online: 7 DEC 2006

DOI: 10.1002/9780470146330.ch6

Mass Spectrometry of Protein Interactions

Mass Spectrometry of Protein Interactions

How to Cite

Maleknia, S. D. and Downard, K. M. (2007) Genesis and Application of Radical Probe Mass Spectrometry (RP-MS) to Study Protein Interactions, in Mass Spectrometry of Protein Interactions (ed K. M. Downard), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470146330.ch6

Editor Information

  1. School of Molecular and Microbial Biosciences, The University of Sydney, Australia

Author Information

  1. 1

    School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, Australia

  2. 2

    School of Molecular and Microbial Biosciences, The University of Sydney, Australia

Publication History

  1. Published Online: 7 DEC 2006
  2. Published Print: 26 JUL 2007

ISBN Information

Print ISBN: 9780471793731

Online ISBN: 9780470146330

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Keywords:

  • radical probe mass spectrometry genesis;
  • amino acid side chain interactions;
  • radical oxidation in protein assembly study

Summary

This chapter contains sections titled:

  • Genesis of Radical Probe Mass Spectrometry

  • The Reactive Residue Side Chains

  • Conditions Important to Radical Probe Mass Spectrometry Experiments

  • Generation of Radicals on Millisecond Timescales

  • Applications of RP-MS to Studies of Protein Interactions

  • Onset of Oxidative Damage and Its Implications for Protein Interactions

  • Application of Radical Oxidation to Study Protein Assemblies

  • Modeling Protein Complexes with Data from RP-MS Experiments

  • Conclusions

  • References