Unit

UNIT 1C.16 Generation, Expansion, and Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) Derived From the Umbilical Cords of Newborns

  1. Richard S. Song1,
  2. Jeanne M. Carroll1,
  3. Lisette Acevedo2,
  4. Dongmei Wu3,
  5. Yang Liu3,
  6. Evan Y. Snyder1,3,4

Published Online: 16 MAY 2014

DOI: 10.1002/9780470151808.sc01c16s29

Current Protocols in Stem Cell Biology

Current Protocols in Stem Cell Biology

How to Cite

Song, R. S., Carroll, J. M., Acevedo, L., Wu, D., Liu, Y. and Snyder, E. Y. 2014. Generation, Expansion, and Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) Derived From the Umbilical Cords of Newborns. Current Protocols in Stem Cell Biology. 29:C:1C.16:1C.16.1–1C.16.13.

Author Information

  1. 1

    Department of Pediatrics, University of California at San Diego and Rady Children's Hospital, San Diego, California

  2. 2

    Moores Cancer Center, University of California at San Diego, La Jolla, California

  3. 3

    Sanford-Burnham Medical Research Institute, La Jolla, California

  4. 4

    Sanford Consortium for Regenerative Medicine, La Jolla, California

Publication History

  1. Published Online: 16 MAY 2014

Abstract

The umbilical cord is tissue that is normally discarded after the delivery of the infant, but it has been shown to be a rich source of stem cells from the cord blood, Wharton's jelly, and umbilical endothelial cells. Patient-specific human induced pluripotent stem cells (hiPSCs) reprogrammed from patient specific human umbilical vein endothelial cells in the neonatal intensive care unit (NICU) population (specifically, premature neonates) have not been shown in the literature. This unit describes a protocol for the generation and expansion of hiPSCs originating from umbilical cords collected from patients in the NICU. Curr. Protoc. Stem Cell Biol. 29:1C.16.1-1C.16.13. © 2014 by John Wiley & Sons, Inc.

Keywords:

  • umbilical cord;
  • HUVEC;
  • reprogramming;
  • iPS