UNIT 1C.16 Generation, Expansion, and Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) Derived From the Umbilical Cords of Newborns
Published Online: 16 MAY 2014
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Stem Cell Biology
How to Cite
Song, R. S., Carroll, J. M., Acevedo, L., Wu, D., Liu, Y. and Snyder, E. Y. 2014. Generation, Expansion, and Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) Derived From the Umbilical Cords of Newborns. Current Protocols in Stem Cell Biology. 29:C:1C.16:1C.16.1–1C.16.13.
- Published Online: 16 MAY 2014
The umbilical cord is tissue that is normally discarded after the delivery of the infant, but it has been shown to be a rich source of stem cells from the cord blood, Wharton's jelly, and umbilical endothelial cells. Patient-specific human induced pluripotent stem cells (hiPSCs) reprogrammed from patient specific human umbilical vein endothelial cells in the neonatal intensive care unit (NICU) population (specifically, premature neonates) have not been shown in the literature. This unit describes a protocol for the generation and expansion of hiPSCs originating from umbilical cords collected from patients in the NICU. Curr. Protoc. Stem Cell Biol. 29:1C.16.1-1C.16.13. © 2014 by John Wiley & Sons, Inc.
- umbilical cord;