UNIT 1D.8 Using ES Cells Labeled with GFP for Analyzing Cell Behavior During Differentiation
Published Online: 1 AUG 2012
Copyright © 2012 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Stem Cell Biology
How to Cite
Liu, Z., Vong, Q. P. and Zheng, Y. 2012. Using ES Cells Labeled with GFP for Analyzing Cell Behavior During Differentiation. Current Protocols in Stem Cell Biology. 22:D:1D.8:1D.8.1–1D.8.9.
- Published Online: 1 AUG 2012
Mouse embryonic stem (ES) cell is a unique yet highly successful system to study stem cell maintenance and differentiation. In this protocol, we describe the generation of engineered mouse ES cells that stably express GFP alone or GFP fused with histone H2B, which allow the cells to be traced live or in fixed samples. This system can be used to study cell behavior changes as ES cells differentiate into different lineages. Curr. Protoc. Stem Cell Biol. 22:1D.8.1-1D.8.9. © 2012 by John Wiley & Sons, Inc.
- mouse embryonic stem cell;
- live imaging;
- time-lapse movie;