Unit

UNIT 1D.8 Using ES Cells Labeled with GFP for Analyzing Cell Behavior During Differentiation

  1. Zhonghua Liu1,
  2. Queenie P. Vong1,2,
  3. Yixian Zheng1

Published Online: 1 AUG 2012

DOI: 10.1002/9780470151808.sc01d08s22

Current Protocols in Stem Cell Biology

Current Protocols in Stem Cell Biology

How to Cite

Liu, Z., Vong, Q. P. and Zheng, Y. 2012. Using ES Cells Labeled with GFP for Analyzing Cell Behavior During Differentiation. Current Protocols in Stem Cell Biology. 22:D:1D.8:1D.8.1–1D.8.9.

Author Information

  1. 1

    Carnegie Institution for Science, Department of Embryology, Baltimore, Maryland

  2. 2

    St. Jude Children's Research Hospital, Memphis, Tennessee

Publication History

  1. Published Online: 1 AUG 2012

Abstract

Mouse embryonic stem (ES) cell is a unique yet highly successful system to study stem cell maintenance and differentiation. In this protocol, we describe the generation of engineered mouse ES cells that stably express GFP alone or GFP fused with histone H2B, which allow the cells to be traced live or in fixed samples. This system can be used to study cell behavior changes as ES cells differentiate into different lineages. Curr. Protoc. Stem Cell Biol. 22:1D.8.1-1D.8.9. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • mouse embryonic stem cell;
  • live imaging;
  • time-lapse movie;
  • differentiation