Unit

UNIT 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells

  1. Koji Hayakawa1,
  2. Emi Himeno1,
  3. Satoshi Tanaka1,
  4. Tilo Kunath2

Published Online: 2 FEB 2015

DOI: 10.1002/9780470151808.sc01e04s32

Lab Protocol Title

Current Protocols in Stem Cell Biology

Current Protocols in Stem Cell Biology

Additional Information

How to Cite

Hayakawa, K. , Himeno, E. , Tanaka, S. , and Kunath, T. 2015. Isolation and Manipulation of Mouse Trophoblast Stem Cells. Curr. Protoc. Stem Cell Biol. 32:1E.4.1-1E.4.32. doi: 10.1002/9780470151808.sc01e04s32

Author Information

  1. 1

    Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The University of Tokyo, Tokyo, Japan

  2. 2

    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh, Edinburgh, United Kingdom

Publication History

  1. Published Online: 2 FEB 2015
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Abstract

The isolation of stable trophoblast stem (TS) cell lines from early mouse embryos has provided a useful cell culture model to study trophoblast development. TS cells are derived from pre-implantation blastocysts or from the extraembryonic ectoderm of early post-implantation embryos. The derivation and maintenance of mouse TS cells is dependent upon continuous fibroblast growth factor (FGF) signaling. Gene expression analysis, differentiation in culture, and chimera formation show that TS cells accurately model the mouse trophoblast lineage. This unit describes how to derive, maintain, and manipulate TS cells, including DNA transfection and chimera formation. © 2015 by John Wiley & Sons, Inc.

Keywords:

  • trophoblast stem cells;
  • TS cells;
  • extraembryonic ectoderm;
  • trophectoderm;
  • trophoblast;
  • FGF4
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