Unit

UNIT 1G.4 Differentiation of Hepatocytes from Pluripotent Stem Cells

  1. Sunil K. Mallanna,
  2. Stephen A. Duncan

Published Online: 20 SEP 2013

DOI: 10.1002/9780470151808.sc01g04s26

Current Protocols in Stem Cell Biology

Current Protocols in Stem Cell Biology

How to Cite

Mallanna, S. K. and Duncan, S. A. 2013. Differentiation of Hepatocytes from Pluripotent Stem Cells. Current Protocols in Stem Cell Biology. 26:G:1G.4:1G.4.1–1G.4.13.

Author Information

  1. Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin

Publication History

  1. Published Online: 20 SEP 2013

ABSTRACT

Differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells into hepatocyte-like cells provides a platform to study the molecular basis of human hepatocyte differentiation, to develop cell culture models of liver disease, and to potentially provide hepatocytes for treatment of end-stage liver disease. Additionally, hepatocyte-like cells generated from human pluripotent stem cells could serve as platforms for drug discovery, determination of pharmaceutical-induced hepatotoxicity, and evaluation of idiosyncratic drug-drug interactions. Here, we describe a step-wise protocol previously developed in our laboratory that facilitates the highly efficient and reproducible differentiation of human pluripotent stem cells into hepatocyte-like cells. Our protocol uses defined culture conditions and closely recapitulates key developmental events that are found to occur during hepatogenesis. Curr. Protoc. Stem Cell Biol. 26:1G.4.1-1G.4.13. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • hepatocytes;
  • liver development;
  • definitive endoderm;
  • iPSC;
  • hESC