Miscellaneous

UNIT 1H.4 Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells

  1. Sara Gualdoni1,
  2. Michael Baron1,
  3. Jörn Lakowski1,
  4. Sarah Decembrini1,
  5. Rachel A. Pearson2,
  6. Robin R. Ali2,
  7. Jane C. Sowden1

Published Online: 1 DEC 2011

DOI: 10.1002/9780470151808.sc01h04s19

Lab Protocol Title

Current Protocols in Stem Cell Biology

Current Protocols in Stem Cell Biology

Additional Information

How to Cite

Gualdoni, S., Baron, M., Lakowski, J., Decembrini, S., Pearson, R. A., Ali, R. R. and Sowden, J. C. 2011. Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. Current Protocols in Stem Cell Biology. 19:1H.4.1–1H.4.15.

Author Information

  1. 1

    Developmental Biology Unit, UCL Institute of Child Health, University College London, London, United Kingdom

  2. 2

    Department of Genetics, UCL Institute of Ophthalmology, University College London, London, United Kingdom

Publication History

  1. Published Online: 1 DEC 2011
  2. Published Print: DEC 2011
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The protocols described in this unit provide detailed information on how to isolate and expand, in culture, ciliary epithelial cells (CECs), previously identified as retinal stem cells, from the adult mouse eye, and embryonic retinal progenitor cells (RPCs) from the developing retina. CECs are initially cultured in floating conditions as neurospheres and then expanded in monolayer cultures. RPCs are cultured in floating conditions. Detailed protocols for retinal differentiation, as well as exogenous gene expression using lentivirus are also described. Curr. Protoc. Stem Cell Biol. 19:1H.4.1-1H.4.15. © 2011 by John Wiley & Sons, Inc.

Keywords: ciliary epithelial cells (CECs); retinal stem cells; retinal progenitor cells (RPCs); monolayer cultures; neurospheres; retinal differentiation

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