UNIT 1H.6 Dopaminergic Differentiation of Human Pluripotent Cells
Published Online: 1 AUG 2012
Copyright © 2012 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Stem Cell Biology
How to Cite
Boyer, L. F., Campbell, B., Larkin, S., Mu, Y. and Gage, F. H. 2012. Dopaminergic Differentiation of Human Pluripotent Cells. Current Protocols in Stem Cell Biology. 22:H:1H.6:1H.6.1–1H.6.11.
- Published Online: 1 AUG 2012
Here we describe protocols for the dopaminergic differentiation of pluripotent stem cells. We have optimized and compared two distinct protocols, both of which are chemically defined and applicable to both embryonic and induced pluripotent stem cells. First, we present a five-step method based on rosette formation (Basic Protocol 1); then we describe a monolayer paradigm based on inhibition of alternate developmental pathways (Basic Protocol 2). Directed differentiation of pluripotent cells into specific cell types is a crucial step towards understanding human development and realizing the biomedical relevance of these cells, whether for replacement therapy or disease modeling. Curr. Protoc. Stem Cell Biol. 22:1H.6.1-1H.6.11. © 2012 by John Wiley & Sons, Inc.
- neural development;
- dopaminergic differentiation;
- pluripotent stem cells