Unit

UNIT 1H.6 Dopaminergic Differentiation of Human Pluripotent Cells

  1. Leah F. Boyer1,2,
  2. Benjamin Campbell1,
  3. Samantha Larkin1,
  4. Yanling Mu1,
  5. Fred H. Gage1

Published Online: 1 AUG 2012

DOI: 10.1002/9780470151808.sc01h06s22

Current Protocols in Stem Cell Biology

Current Protocols in Stem Cell Biology

How to Cite

Boyer, L. F., Campbell, B., Larkin, S., Mu, Y. and Gage, F. H. 2012. Dopaminergic Differentiation of Human Pluripotent Cells. Current Protocols in Stem Cell Biology. 22:H:1H.6:1H.6.1–1H.6.11.

Author Information

  1. 1

    Salk Institute for Biological Studies, San Diego, California

  2. 2

    Biomedical Sciences Graduate Program, School of Medicine, University of California, San Diego, California

Publication History

  1. Published Online: 1 AUG 2012

Abstract

Here we describe protocols for the dopaminergic differentiation of pluripotent stem cells. We have optimized and compared two distinct protocols, both of which are chemically defined and applicable to both embryonic and induced pluripotent stem cells. First, we present a five-step method based on rosette formation (Basic Protocol 1); then we describe a monolayer paradigm based on inhibition of alternate developmental pathways (Basic Protocol 2). Directed differentiation of pluripotent cells into specific cell types is a crucial step towards understanding human development and realizing the biomedical relevance of these cells, whether for replacement therapy or disease modeling. Curr. Protoc. Stem Cell Biol. 22:1H.6.1-1H.6.11. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • neural development;
  • dopaminergic differentiation;
  • pluripotent stem cells