Unit

UNIT 2B.5 Preparation and Osteogenic Differentiation of Scaffold-Free Mouse Adipose-Derived Stromal Cell Microtissue Spheroids (ASC-MT)

  1. Ali Mirsaidi,
  2. André N. Tiaden,
  3. Peter J. Richards

Published Online: 13 NOV 2013

DOI: 10.1002/9780470151808.sc02b05s27

Current Protocols in Stem Cell Biology

Current Protocols in Stem Cell Biology

How to Cite

Mirsaidi, A., Tiaden, A. N. and Richards, P. J. 2013. Preparation and Osteogenic Differentiation of Scaffold-Free Mouse Adipose-Derived Stromal Cell Microtissue Spheroids (ASC-MT). Current Protocols in Stem Cell Biology. 27:B:2B.5:2B.5.1–2B.5.12.

Author Information

  1. Bone and Stem Cell Research Group, CABMM, University of Zurich, Zurich, Switzerland

Publication History

  1. Published Online: 13 NOV 2013

Abstract

In this unit, previously described methods are expanded upon, where procedures relating to the preparation, culturing, and osteogenic differentiation of scaffold-free mouse adipose-derived stromal cell microtissue spheroids (ASC-MT) are outlined. Not only is a detailed methodology of how to engineer such spheroids are presented, but a full account of how to induce and analyze osteogenesis in these ASC-MT constructs is given along with relevant figures to help better illustrate the methods described. Curr. Protoc. Stem Cell Biol. 27:2B.5.1-2B.5.12. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • adipose-derived stromal cells;
  • osteogenesis;
  • 3D-culture