UNIT 2B.5 Preparation and Osteogenic Differentiation of Scaffold-Free Mouse Adipose-Derived Stromal Cell Microtissue Spheroids (ASC-MT)
Published Online: 13 NOV 2013
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Stem Cell Biology
How to Cite
Mirsaidi, A., Tiaden, A. N. and Richards, P. J. 2013. Preparation and Osteogenic Differentiation of Scaffold-Free Mouse Adipose-Derived Stromal Cell Microtissue Spheroids (ASC-MT). Current Protocols in Stem Cell Biology. 27:B:2B.5:2B.5.1–2B.5.12.
- Published Online: 13 NOV 2013
In this unit, previously described methods are expanded upon, where procedures relating to the preparation, culturing, and osteogenic differentiation of scaffold-free mouse adipose-derived stromal cell microtissue spheroids (ASC-MT) are outlined. Not only is a detailed methodology of how to engineer such spheroids are presented, but a full account of how to induce and analyze osteogenesis in these ASC-MT constructs is given along with relevant figures to help better illustrate the methods described. Curr. Protoc. Stem Cell Biol. 27:2B.5.1-2B.5.12. © 2013 by John Wiley & Sons, Inc.
- adipose-derived stromal cells;