Unit

UNIT 2D.12 Scalable Production of Transplantable Dopaminergic Neurons from hESCs and iPSCs in Xeno-Free Defined Conditions

  1. Andrzej Swistowski1,2,
  2. Xianmin Zeng1

Published Online: 1 AUG 2012

DOI: 10.1002/9780470151808.sc02d12s22

Current Protocols in Stem Cell Biology

Current Protocols in Stem Cell Biology

How to Cite

Swistowski, A. and Zeng, X. 2012. Scalable Production of Transplantable Dopaminergic Neurons from hESCs and iPSCs in Xeno-Free Defined Conditions. Current Protocols in Stem Cell Biology. 22:D:2D.12:2D.12.1–2D.12.20.

Author Information

  1. 1

    Buck Institute for Age Research, Novato, California

  2. 2

    XCell Science Inc., Novato, California

Publication History

  1. Published Online: 1 AUG 2012

Abstract

Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) are potentially an unlimited cell source for cell replacement therapy and personalized medicine. Before hESC- and iPSC-based therapy can be moved from bench to bedside, however, it is essential to establish protocols for generating therapeutically relevant cells, like dopaminergic neurons in defined conditions that are suitable for scalable good manufacturing practice (GMP)-compliant protocols. Here, the derivation and differentiation of functional dopaminergic neurons from hESCs and iPSCs under xeno-free defined conditions are described. These protocols have been validated in multiple hESC and iPSC lines. Curr. Protoc. Stem Cell Biol. 22:2D.12.1-2D.12.20. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • human ESCs;
  • human iPSCs;
  • xeno-free;
  • neural stem cell;
  • dopaminergic neuron