Unit

UNIT 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells

  1. Mari Ohnuki1,2,
  2. Kazutoshi Takahashi1,
  3. Shinya Yamanaka1,2,3,4

Published Online: 1 JUN 2009

DOI: 10.1002/9780470151808.sc04a02s9

Current Protocols in Stem Cell Biology

Current Protocols in Stem Cell Biology

How to Cite

Ohnuki, M., Takahashi, K. and Yamanaka, S. 2009. Generation and Characterization of Human Induced Pluripotent Stem Cells. Current Protocols in Stem Cell Biology. 9:A:4A.2:4A.2.1–4A.2.25.

Author Information

  1. 1

    Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto, Japan

  2. 2

    Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan

  3. 3

    Japan Science and Technology Agency, Kawaguchi, Japan

  4. 4

    Gladstone Institute of Cardiovascular Disease, San Francisco, California

Publication History

  1. Published Online: 1 JUN 2009
  2. Published Print: SEP 2009

Abstract

This unit describes how to generate human induced pluripotent stem (iPS) cells and evaluate the qualities of the generated iPS cells. The methods for establishment and maintenance of human iPS cells are similar to those for mouse iPS cells but not identical. In addition, these protocols include excellent procedures for passaging and cryopreservation of human iPS cells established by ES cell researchers, which result in an easy way to culture human iPS cells. Moreover, we include methods for characterizing iPS cells for further research. RT-PCR and immunocytochemistry for detection of pluripotent cell markers, embryoid body differentiation, and teratoma differentiation are used to determine pluripotency in vitro and in vivo, respectively. Curr. Protoc. Stem Cell Biol. 9:4A.2.1-4A.2.25. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • reprogramming;
  • pluripotency;
  • iPS cells