1. Preparation and Bioactive Characteristics of Porous Borate Glass Substrates

  1. Mineo Mizuno
  1. Mohamed N. Rahaman,
  2. Wen Liang and
  3. Delbert E. Day

Published Online: 27 MAR 2008

DOI: 10.1002/9780470291269.ch1

Advances in Bioceramics and Biocomposites: Ceramic Engineering and Science Proceedings, Volume 26, Number 6

Advances in Bioceramics and Biocomposites: Ceramic Engineering and Science Proceedings, Volume 26, Number 6

How to Cite

Rahaman, M. N., Liang, W. and Day, D. E. (2005) Preparation and Bioactive Characteristics of Porous Borate Glass Substrates, in Advances in Bioceramics and Biocomposites: Ceramic Engineering and Science Proceedings, Volume 26, Number 6 (ed M. Mizuno), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470291269.ch1

Author Information

  1. University of Missouri-Rolla, Department of Materials Science and Engineering, and Materials Research Center, Rolla, MO 65409

Publication History

  1. Published Online: 27 MAR 2008
  2. Published Print: 1 JAN 2005

ISBN Information

Print ISBN: 9781574982367

Online ISBN: 9780470291269

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Keywords:

  • osteoblasts;
  • phosphatase;
  • biocompatiile;
  • engineering;
  • microscopy

Summary

Whereas silicate-based bioactive glasses and glass-ceramics have been widely investigated for bone repair or as scaffolds for cell-based bone tissue engineering, recent data have demonstrated that silica-free borate glasses also exhibit bioactive behavior. The objectives of this study were to fabricate porous, three-dimensional substrates of a borate glass and to investigate the biocompatibility of the borate glass substrates by in vitro cell culture with human mesenchymal stem cells (hMSCs) and hMSC-derived osteoblasts (hMSC-Obs). Borate glass particles with sizes 212–355 μm were loosely compacted and then sintered at 600°C to form porous discshaped substrates (porosity ∽ 40%). Partial or nearly complete conversion of the glass substrates to a calcium phosphate (Ca-P) material was achieved by soaking the substrates for 1 day or 7 days in a 0.25 molar K2HPO4 solution at 37°C and at pH of 9.0. Bone marrow derived hMSCs and hMSC-Obs seeded in the samples both adhered to the porous constructs whereas hMSC-Obs markedly synthesized alkaline phosphatase, an early osteogenic marker. These data indicate strong bioactive characteristics for the borate glass constructs and the potential use of the constructs for bone tissue engineering.