14. In Vitro Comparison of the Apatite Inducing Ability of Three Different SBF Solutions on Ti6A14V

  1. Mineo Mizuno
  1. Sahil Jalota,
  2. A. Cuneyt Tas and
  3. Sarit B. Bhaduri

Published Online: 27 MAR 2008

DOI: 10.1002/9780470291269.ch14

Advances in Bioceramics and Biocomposites: Ceramic Engineering and Science Proceedings, Volume 26, Number 6

Advances in Bioceramics and Biocomposites: Ceramic Engineering and Science Proceedings, Volume 26, Number 6

How to Cite

Jalota, S., Tas, A. C. and Bhaduri, S. B. (2005) In Vitro Comparison of the Apatite Inducing Ability of Three Different SBF Solutions on Ti6A14V, in Advances in Bioceramics and Biocomposites: Ceramic Engineering and Science Proceedings, Volume 26, Number 6 (ed M. Mizuno), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470291269.ch14

Author Information

  1. School of Materials Science and Engineering, Clemson University, Clemson, SC 29634, USA

Publication History

  1. Published Online: 27 MAR 2008
  2. Published Print: 1 JAN 2005

ISBN Information

Print ISBN: 9781574982367

Online ISBN: 9780470291269

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Keywords:

  • phosphates;
  • physiological;
  • biomimetic;
  • morphology;
  • ostcoblasts

Summary

Coating of titanium-based biomedical devices with a layer of carbonated, apatitic calcium phosphate (CaP) increases their bone-bonding ability. Synthetic or simulated body fluids (SBF) have the ability of forming apatitic calcium phosphates on the immersed titanium alloys within few days to 2 weeks. Apatite-inducing ability of 5 M NaOH-etched surfaces of Ti6A14V strips (10 × 10 × 1 mm) were tested by using three different SBF solutions all concentrated by a factor of 1. 5. SBF solutions used in this comparative study were i) 4. 2 mM HCO3-- TRIS-HC1 buffered SBF (conventional SBF or c-SBF), ii) 27 mM HCO3-- TRIS-HCl buffered SBF (Tas-SBF), and iii) 27 mM HCO3-- HEPES-NaOH buffered SBF (revised SBF or r-SBF). c-SBF (4. 2 mM HCO3--) was quite slow in forming CaP on Ti6A14V strips after 1 week of soaking at 37°C, whereas TasSBF of 27 mM HCO3-- was able to fully coat the immersed samples. Cell viability, protein concentration and cell attachment were tested on the coated Ti6A14V strips by using mouse osteoblasts (7F2).