15. In Situ and Long Term Evaluation of Calcium Phosphate Cement Behavior in Animal Experiment
- Mineo Mizuno
Published Online: 27 MAR 2008
Copyright © 2005 The American Ceramic Society
Advances in Bioceramics and Biocomposites: Ceramic Engineering and Science Proceedings, Volume 26, Number 6
How to Cite
Mukaida, M., Neo, M., Nakamura, T., Mizuta, Y., Ikceda, Y. and Mizuno, M. (2005) In Situ and Long Term Evaluation of Calcium Phosphate Cement Behavior in Animal Experiment, in Advances in Bioceramics and Biocomposites: Ceramic Engineering and Science Proceedings, Volume 26, Number 6 (ed M. Mizuno), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470291269.ch15
- Published Online: 27 MAR 2008
- Published Print: 1 JAN 2005
Print ISBN: 9781574982367
Online ISBN: 9780470291269
High-resolution X-ray CT is a powerful means of analyzing a comprehensive range of ceramic biomaterials in vivo. The benefit of this method is that morphological and volume changes of implant materials can be evaluated without retrieval of the implant, allowing animal viability to be maintained and allowing long-term repeated evaluation.
In this study, in situ techniques for the observation of calcium phosphate cement (CPC) were developed. CPC was implanted into the femur and under the skin of rats. The volume and morphology change of the CPC were repeatedly measured in the same rats for more than 12 months.
The structure of the CPC was visualized in three dimensions (3-D), and its volume was quantified using 3-D structure analysis software, which enabled two-value processing and estimation of the quantities of the CPC. Moreover, some CPC samples were retrieved and observed by SEM.
The surface of the CPC changed from smooth to jagged as time increased. The volume of CPC implanted into bone gradually decreased with time. The volume loss was 8% after 12 months. The volume of the CPC implanted subcutaneously increased by 7% in one month, and subsequently decreased gradually.
HRXCT was found to be a powerful means for analyzing biomaterials such as porous ceramics and bone cements in vivo.