Chapter 44. Cell-Free Expression System for Eukaryotic Proteins

  1. Ganesh Kumar Agrawal2 and
  2. Randeep Rakwal3,4,5
  1. Yaeta Endo and
  2. Tatsuya Sawasaki

Published Online: 7 JAN 2008

DOI: 10.1002/9780470369630.ch44

Plant Proteomics: Technologies, Strategies, and Applications

Plant Proteomics: Technologies, Strategies, and Applications

How to Cite

Endo, Y. and Sawasaki, T. (2008) Cell-Free Expression System for Eukaryotic Proteins, in Plant Proteomics: Technologies, Strategies, and Applications (eds G. K. Agrawal and R. Rakwal), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470369630.ch44

Editor Information

  1. 2

    Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal

  2. 3

    Research Laboratory for Agricultural Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal

  3. 4

    Health Technology, Research Center (HTRC), Ibaraki, Japan

  4. 5

    National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba West, Ibaraki, Japan

Author Information

  1. Cell-Free Science and Technology Research Center, Ehime University, Matsuyama, Japan

Publication History

  1. Published Online: 7 JAN 2008
  2. Published Print: 27 AUG 2008

ISBN Information

Print ISBN: 9780470069769

Online ISBN: 9780470369630

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Keywords:

  • continuous-flow cell-free (CFCF) translation method;
  • wheat germ cell-free translation system;
  • germ cell-free translation and eukaryotic multidomain protein

Summary

This chapter contains sections titled:

  • Introduction

  • Development of Wheat Germ Cell-Free Protein Synthesis System

  • Application to Functional Proteomics

  • Application to Structural Proteomics

  • Conclusions and Five-Year Viewpoint

  • References