Chapter 12. Polarographic Oxygen Sensors, the Oxygraph, and High-Resolution Respirometry to Assess Mitochondrial Function

  1. James A. Dykens3,
  2. Yvonne Will4
  1. Erich Gnaiger1,2

Published Online: 22 JAN 2008

DOI: 10.1002/9780470372531.ch12

Book Title

Drug-Induced Mitochondrial Dysfunction

Drug-Induced Mitochondrial Dysfunction

Additional Information

How to Cite

Gnaiger, E. (2008) Polarographic Oxygen Sensors, the Oxygraph, and High-Resolution Respirometry to Assess Mitochondrial Function, in Drug-Induced Mitochondrial Dysfunction (eds J. A. Dykens and Y. Will), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470372531.ch12

Editor Information

  1. 3

    Pfizer, Inc., Sandwich, UK

  2. 4

    Pfizer, Inc., Groton, Connecticut, USA

Author Information

  1. 1

    Department of General and Transplant Surgery, D. Swarovski Research Laboratory, Medical University of Innsbruck, Innsbruck, Austria

  2. 2

    OROBOROS INSTRUMENTS, Innsbruck, Austria

Publication History

  1. Published Online: 22 JAN 2008
  2. Published Print: 14 SEP 2008

ISBN Information

Print ISBN: 9780470111314

Online ISBN: 9780470372531

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CHAPTER TOOLS

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Keywords:

  • polarographic oxygen sensor and traditional oxygraphy;
  • polarographic oxygen sensors (OROBoPOS);
  • phosphorylation control protocol (PC protocol)

Summary

This chapter contains sections titled:

  • Introduction

  • Polarographic oxygen sensor and traditional oxygraphy

  • High-resolution respirometry: the Oxygraph-2k

  • Phosphorylation control protocol with intact cells

  • Intact cells, permeabilized cells and tissue, or isolated mitochondria

  • Titration protocols in permeabilized cells, permeabilized tissue preparations, and isolated mitochondria

  • Multisensor applications

  • References

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