Chapter 17. Use of Fluorescent Reporters to Measure Mitochondrial Membrane Potential and the Mitochondrial Permeability Transition

  1. James A. Dykens4,
  2. Yvonne Will5
  1. Anna-Liisa Nieminen1,2,
  2. Venkat K. Ramshesh1,2,
  3. John J. Lemasters1,3

Published Online: 22 JAN 2008

DOI: 10.1002/9780470372531.ch17

Book Title

Drug-Induced Mitochondrial Dysfunction

Drug-Induced Mitochondrial Dysfunction

Additional Information

How to Cite

Nieminen, A.-L., Ramshesh, V. K. and Lemasters, J. J. (2008) Use of Fluorescent Reporters to Measure Mitochondrial Membrane Potential and the Mitochondrial Permeability Transition, in Drug-Induced Mitochondrial Dysfunction (eds J. A. Dykens and Y. Will), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470372531.ch17

Editor Information

  1. 4

    Pfizer, Inc., Sandwich, UK

  2. 5

    Pfizer, Inc., Groton, Connecticut, USA

Author Information

  1. 1

    Center for Cell Death, Injury and Regeneration, Charleston, South Carolina, USA

  2. 2

    Department of Pharmaceutical and Biomedical Sciences, Hollings Cancer Center, Medical University of South Carolina, Charleston, South Carolina, USA

  3. 3

    Departments of Pharmaceutical and Biomedical Sciences and Biochemistry and Molecular Biology, Hollings Cancer Center, Medical University of South Carolina, Charleston, South Carolina, USA

Publication History

  1. Published Online: 22 JAN 2008
  2. Published Print: 14 SEP 2008

ISBN Information

Print ISBN: 9780470111314

Online ISBN: 9780470372531

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Keywords:

  • mitochondrial isolation and fluorescent probes;
  • fluorescent reporter use;
  • plasma membrane permeability and calcein

Summary

This chapter contains sections titled:

  • Introduction

  • Isolated mitochondria

  • Nernstian distribution of fluorescent probes

  • Monitoring membrane potential in isolated mitochondria

  • Imaging Δψ in single intact cells

  • Mitochondrial permeability transition

  • Visualizing the MPT in intact cells

  • Plasma membrane permeability

  • Conclusions

  • References

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