2. Crystallization of Macromolecules

  1. Alexander McPherson

Published Online: 11 MAR 2008

DOI: 10.1002/9780470391518.ch2

Introduction to Macromolecular Crystallography, Second Edition

Introduction to Macromolecular Crystallography, Second Edition

How to Cite

McPherson, A. (2009) Crystallization of Macromolecules, in Introduction to Macromolecular Crystallography, Second Edition, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470391518.ch2

Author Information

  1. University of California, USA

Publication History

  1. Published Online: 11 MAR 2008
  2. Published Print: 16 JAN 2009

ISBN Information

Print ISBN: 9780470185902

Online ISBN: 9780470391518

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Keywords:

  • macromolecule crystallization ;
  • protein crystal growth influencing factors;
  • crystallization strategy

Summary

This chapter contains sections titled:

  • Crystals Grow from Supersaturated Solutions

  • Why Crystals Grow

  • Proteins Present Special Problems for Crystallographers

  • Properties of Macromolecular Crystals

  • Crystallization Strategy

  • Screening and Optimization

  • Creating the Supersaturated State

  • Precipitating Agents

  • Factors Influencing Protein Crystal Growth

  • Some Useful Considerations

  • Typical Trial Arrays

  • The Importance of Protein Purity and Homogeneity

  • Solubilization

  • Seeding

  • Automated Crystallization and Robotics

  • Important Principles