Chapter 7. Optimizing Sensitivity and Specificity in Mass Spectrometric Proteome Analysis

  1. Rolf Ekman3,
  2. Jerzy Silberring4,
  3. Ann Westman-Brinkmalm3 and
  4. Agnieszka Kraj4
  1. Jan Eriksson1 and
  2. David Fenyö2

Published Online: 26 MAR 2008

DOI: 10.1002/9780470395813.ch7

Mass Spectrometry: Instrumentation, Interpretation, and Applications

Mass Spectrometry: Instrumentation, Interpretation, and Applications

How to Cite

Eriksson, J. and Fenyö, D. (2008) Optimizing Sensitivity and Specificity in Mass Spectrometric Proteome Analysis, in Mass Spectrometry: Instrumentation, Interpretation, and Applications (eds R. Ekman, J. Silberring, A. Westman-Brinkmalm and A. Kraj), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470395813.ch7

Editor Information

  1. 3

    Institute of Neuroscience and Physiology, The Sahlgrenska Academy, University of Gothenburg, Molndal, Sweden

  2. 4

    Poland Faculty of Chemistry and Regional Laboratory, Jagiellonian University, Krakow, Poland

Author Information

  1. 1

    Department of Chemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden

  2. 2

    The Rockefeller University, New York, New York, USA

Publication History

  1. Published Online: 26 MAR 2008
  2. Published Print: 21 NOV 2008

Book Series:

  1. Wiley-Interscience Series in Mass Spectrometry

Book Series Editors:

  1. Dominic M. Desiderio5,6 and
  2. Nico M. M. Nibbering7

Series Editor Information

  1. 5

    Departments of Neurology and Biochemistry, USA

  2. 6

    University of Tennessee Health Science Center, USA

  3. 7

    Vrije Universiteit Amsterdam, The Netherlands

ISBN Information

Print ISBN: 9780471713951

Online ISBN: 9780470395813

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Keywords:

  • MS proteome analysis and optimizing sensitivity and specificity;
  • success rate and relative dynamic range;
  • computation and simulation tools

Summary

This chapter contains sections titled:

  • Quantitation

  • Peptide and Protein Identification

  • Success Rate and Relative Dynamic Range

  • Summary

  • References