13. Media and Extra Cellular Matrix Requirements for Large-Scale ESC Growth

  1. Uma Lakshmipathy,
  2. Jonathan D. Chesnut and
  3. Bhaskar Thyagarajan
  1. Allan J. Robins and
  2. Thomas C. Schulz

Published Online: 17 APR 2009

DOI: 10.1002/9780470454923.ch13

Emerging Technology Platforms for Stem Cells

Emerging Technology Platforms for Stem Cells

How to Cite

Robins, A. J. and Schulz, T. C. (2009) Media and Extra Cellular Matrix Requirements for Large-Scale ESC Growth, in Emerging Technology Platforms for Stem Cells (eds U. Lakshmipathy, J. D. Chesnut and B. Thyagarajan), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470454923.ch13

Editor Information

  1. Primary and Stem Cell Systems, Invitrogen Corporation, 5781 Faraday Avenue, Carlsbad, CA 92008, USA

Author Information

  1. Novocell Inc., 111 Riverbend Road, Athens, GA, 30605, USA

Publication History

  1. Published Online: 17 APR 2009
  2. Published Print: 18 MAR 2009

ISBN Information

Print ISBN: 9780470146934

Online ISBN: 9780470454923

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Keywords:

  • Human embryonic stem cells (hESCs) and media and extracellular matrix requirements;
  • optimized hESC culture system properties;
  • pluripotent hESCs - offering promise of revolutionary cellular therapies

Summary

This chapter contains sections titled:

  • Introduction

  • Properties of an Optimized HESC Culture System

  • Receptors, Signaling, and Self-Renewal

  • Development of DC-Haif Medium

  • Robust Single Cell Passaging of HESCS

  • Scaled Expansion of HESCS

  • Batch Quality-Assurance Testing

  • Summary

  • Methods for Single Cell Passaging and Scaled Expansion of hESCs

  • Acknowledgments

  • Note

  • References