19. Sleeping Beauty-Mediated Transposition in Stem Cells

  1. Uma Lakshmipathy,
  2. Jonathan D. Chesnut and
  3. Bhaskar Thyagarajan
  1. Andrew Wilber1,2,3,
  2. Jakub Tolar4,
  3. Bruce R. Blazar4,
  4. Catherine M. Verfaillie5,6,
  5. Uma Lakshmipathy5,7,8,
  6. Dan S. Kaufman5 and
  7. R. Scott McIvor1

Published Online: 17 APR 2009

DOI: 10.1002/9780470454923.ch19

Emerging Technology Platforms for Stem Cells

Emerging Technology Platforms for Stem Cells

How to Cite

Wilber, A., Tolar, J., Blazar, B. R., Verfaillie, C. M., Lakshmipathy, U., Kaufman, D. S. and McIvor, R. S. (2009) Sleeping Beauty-Mediated Transposition in Stem Cells, in Emerging Technology Platforms for Stem Cells (eds U. Lakshmipathy, J. D. Chesnut and B. Thyagarajan), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470454923.ch19

Editor Information

  1. Primary and Stem Cell Systems, Invitrogen Corporation, 5781 Faraday Avenue, Carlsbad, CA 92008, USA

Author Information

  1. 1

    Beckman Center for Transposon Research, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis MN 55455, USA

  2. 2

    Beckman Center for Transposon Research, Southern Illinois University, Springfield, IL 62794, USA

  3. 3

    Department of Surgery, Southern Illinois University, Springfield IL, 62794, USA

  4. 4

    Pediatric Blood and Marrow Transplant Program, Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA

  5. 5

    Department of Medicine and Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA

  6. 6

    Catholic University, Leuven, Belgium

  7. 7

    Invitrogen Corporation, USA

  8. 8

    Primary and Stem Cell Systems, Invitrogen Corporation, 5781 Faraday Avenue, Carlsbad, CA 92008, USA

Publication History

  1. Published Online: 17 APR 2009
  2. Published Print: 18 MAR 2009

ISBN Information

Print ISBN: 9780470146934

Online ISBN: 9780470454923

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Keywords:

  • sleeping beauty-mediated transposition in stem cells;
  • SB-mediated transposition in embryonic stem (ES) cells;
  • SB transposon system providing nonviral means of integrating new DNA sequences

Summary

This chapter contains sections titled:

  • Introduction

  • Transgenesis of MSC by Transposition

  • SB-Mediated Transposition in MAPC

  • SB-Mediated Transposition in Embryonic Stem (ES) Cells

  • Conclusion

  • References