In vitro Expression of Cloned PRP cDNA Derived from Scrapie-Infected Mouse Brain: Lack of Transmission of Scrapie Infectivity

  1. Greg Bock Organizer and
  2. Joan Marsh
  1. Byron Caughey1,
  2. Richard Race1,
  3. Mari Vogel1,
  4. Michael Buchmeier2 and
  5. Bruce Chesebro1

Published Online: 28 SEP 2007

DOI: 10.1002/9780470513613.ch13

Ciba Foundation Symposium 135 - Novel Infectious Agents and the Central Nervous System

Ciba Foundation Symposium 135 - Novel Infectious Agents and the Central Nervous System

How to Cite

Caughey, B., Race, R., Vogel, M., Buchmeier, M. and Chesebro, B. (2007) In vitro Expression of Cloned PRP cDNA Derived from Scrapie-Infected Mouse Brain: Lack of Transmission of Scrapie Infectivity, in Ciba Foundation Symposium 135 - Novel Infectious Agents and the Central Nervous System (eds G. Bock and J. Marsh), John Wiley & Sons, Ltd., Chichester, UK. doi: 10.1002/9780470513613.ch13

Author Information

  1. 1

    Laboratory of Persistent Viral Diseases, NIAID, NIH, Rocky Mountain Laboratories, Hamilton, Montana 59840, USA

  2. 2

    Department of Immunology, Scripps Clinic and Research Foundation, La Jolla, California, USA

Publication History

  1. Published Online: 28 SEP 2007

ISBN Information

Print ISBN: 9780471915126

Online ISBN: 9780470513613

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Keywords:

  • in vitro expression;
  • cloned PRP CDNA;
  • scrapie-infected mouse brain;
  • scrapie infectivity;
  • formaldehyde

Summary

A cDNA for the prion protein (PrP) derived from scrapie-infected mouse brain was expressed in C127 mouse cells in vitro under the control of the mouse metallothionein promoter. PrP synthesis was detected by immunoprecipitation using a rabbit antibody specific for a 15 amino acid PrP peptide. Homogenates of cells expressing the cloned PrP cDNA inoculated into weanling mice failed to induce clinical scrapie during 190 days of observation. We conclude that either PrP is not the transmissible agent of scrapie or the PrP is not processed appropriately in this cell system to create the infectious agent.