Cell Lineage and Patterns of Migration in the Developing Cortex

  1. Gregory R. Bock Organizer and
  2. Gail Cardew
  1. C. Walsh1 and
  2. C. Reid2

Published Online: 28 SEP 2007

DOI: 10.1002/9780470514795.ch2

Ciba Foundation Symposium 193 - Development of the Cerebral Cortex

Ciba Foundation Symposium 193 - Development of the Cerebral Cortex

How to Cite

Walsh, C. and Reid, C. (2007) Cell Lineage and Patterns of Migration in the Developing Cortex, in Ciba Foundation Symposium 193 - Development of the Cerebral Cortex (eds G. R. Bock and G. Cardew), John Wiley & Sons, Ltd., Chichester, UK. doi: 10.1002/9780470514795.ch2

Author Information

  1. 1

    Neurogenetics Laboratory, Beth Israel Hospital/Harvard Medical School, Alpert 347, 200 Longwood Avenue, Boston, MA 02115, USA

  2. 2

    Washington University School of Medicine, St Louis, MO 63110, USA

Publication History

  1. Published Online: 28 SEP 2007

ISBN Information

Print ISBN: 9780471957058

Online ISBN: 9780470514795

SEARCH

Keywords:

  • cell lineage;
  • migration patterns;
  • histological analysis;
  • sequential formation;
  • clustered clone

Summary

Knowledge of cell lineage in the cortex is important for understanding normal development as well as brain malformations. We studied cell lineage in rats by injecting a library of up to 3400 retroviruses, distinguishable by PCR analysis and encoding alkaline phosphatase, at E14–19. Histological analysis at P15 revealed normal cell morphology and allowed identification of about 80% of all labelled cells. PCR amplification of DNA tags allowed clonal analysis. Cortical cells labelled at E15 formed clustered or widespread clones with equal frequency. Clustered clones contained one to four cells within about 1 mm that had similar morphology and laminar location. However, 48% of cortical clones contained multiple cell types with widely different locations (2.1–6.7 mm; mean, 3.8 mm). Widespread clones contained two to four ‘subunits’ (one to five neurons each), spaced at apparent intervals of 2–3 mm, with each subunit morphologically indistinguishable from a clustered clone. Distinct subunits in the same clone usually differed in laminar location suggesting sequential formation. Clones labelled at E17 contained fewer neurons and up to two subunits. Clustered clones seem to be produced by stationary progenitors, whereas progenitors of clusters may themselves be produced by migratory, multipotential cells.