The Cel Lineage of Neuronal Subtypes in the Mammalian Cerebral Cortex

  1. Gregory R. Bock Organizer and
  2. Gail Cardew
  1. J. G. Parnavelas,
  2. M. C. Mione and
  3. A. Lavdas

Published Online: 28 SEP 2007

DOI: 10.1002/9780470514795.ch3

Ciba Foundation Symposium 193 - Development of the Cerebral Cortex

Ciba Foundation Symposium 193 - Development of the Cerebral Cortex

How to Cite

Parnavelas, J. G., Mione, M. C. and Lavdas, A. (2007) The Cel Lineage of Neuronal Subtypes in the Mammalian Cerebral Cortex, in Ciba Foundation Symposium 193 - Development of the Cerebral Cortex (eds G. R. Bock and G. Cardew), John Wiley & Sons, Ltd., Chichester, UK. doi: 10.1002/9780470514795.ch3

Author Information

  1. Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK

Publication History

  1. Published Online: 28 SEP 2007

ISBN Information

Print ISBN: 9780471957058

Online ISBN: 9780470514795

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Keywords:

  • cell lineage;
  • mammalian cerebral cortex;
  • cortical cell lineages;
  • immunohistochemistry;
  • electron microscopy

Summary

We have studied the lineage relationships of pyramidal and non-pyramidal neurons, the principal neuronal types in the cerebral cortex, using a recombinant retrovirus that carries the gene encoding Escherichiu coli β-galactosidase as a lineage marker. The phenotype of every cell of clones of β-galactosidase-labelled neurons generated by intraventricular injection of recombinant retrovirus in rat embryos at different stages of cortical neurogenesis was identified using light and electron microscopy as well as immunohistochemistry for known markers of neuronal subtypes. We found that clonally related neurons in adult rats showed the same morphological and neurotransmitter phenotypes, suggesting that lineages of pyramidal and non-pyramidal neurons are specified as early as E14, the time of onset of neurogenesis. However, when we followed the development of cortical cell lineages, we noted that a significant number of neuronal clones showed a mixed pyramidal/non-pyramidal cell composition during the first three weeks of life. We suggest that the change in the composition of neuronal clones between the third week of postnatal life and adulthood may either be due to changes in the phenotype of some developing neurons or, more likely, to selective cell death.